Comparison of fluorescence intensity of Hoechst 33342 – stained EMT6 tumour cells and tumour-infiltrating host cells
نویسندگان
چکیده
منابع مشابه
Inducible Fluorescence of Tumour Cells
An oxazine dye, Nile Blue sulfate, induces a fluorescent reaction in cytoplasmic granules (NBIFG) of living or fixed tumour cells in the same manner as reported before for Thiazine dyes. The NBIFG correspond to phase contrast positive granules when cells are viewed by phase contrast microscopy. Fluorescence disappears from NBIFG in a matter of 2 —4 days and the bodies turn deep blue. These gran...
متن کاملCORRELATION BETWEEN EXPRESSION OF CLASS I ANTIGEN AND DEVELOPMENT OF TUMOUR INFILTRATING LYMPHOCYTES (TILS) FROM TUMOUR BIOPSIES OF PATIENTS WITH BLADDER CANCER
This report presents the findings of TIL development from tumour biopsies of patients with bladder cancer. Analysis of tissue sections showed the presence of T cells in intra-epithelial layer in 8 of 12 cases investigated. In a larger group of patients, TILs were established from 7 of 20 cases and expansion of these cells in Interleukin-2 (IL-2) in the presence of conditioned medium (CM) r...
متن کاملInteraction between tumour-infiltrating B cells and T cells controls the progression of hepatocellular carcinoma
OBJECTIVE The nature of the tumour-infiltrating leucocytes (TILs) is known to impact clinical outcome in carcinomas, including hepatocellular carcinoma (HCC). However, the role of tumour-infiltrating B cells (TIBs) remains controversial. Here, we investigate the impact of TIBs and their interaction with T cells on HCC patient prognosis. DESIGN Tissue samples were obtained from 112 patients wi...
متن کاملCross-linking of histone proteins to DNA by UV illumination of chromatin stained with Hoechst 33342.
The photochemical effects of near-UV light on chromatin labeled with the vital DNA dye Hoechst 33342 (H33342) are studied. Several types of experiments demonstrate that illumination at both 365 and 410 nm results in significant cross-linking of proteins with the DNA. Fluorescence microscopy of dye-stained Xenopus XTC-2 nuclei shows that UV illumination has effects similar to chemical fixation b...
متن کاملEvaluation of a side population of canine lymphoma cells using Hoechst 33342 dye
Cancer stem cell (CSC) research has increased exponentially to gain further insight into the mechanisms underlying both carcinogenesis and chemotherapy resistance. The present study was performed to explore the potential value of a side population (SP) assay for identifying and characterizing putative CSCs among canine lymphoma cells. Canine lymphoma cells from cell lines and clinical samples w...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1987
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1987.243